I ahve the same problem on this thread
2000 4R70W intermittent no Drive(3rd) on upshift.
I have a stock 2000 F150 4.2 auto, with 3.55 rear-end. The transmission on an intermittant basis has no 3rd gear.
It shifts perfect from 1st to 2nd, then when going into third its just like it has gone straight into neutral. By backing off the throttle and waiting a couple of seconds it then shifts into 4th/Overdrive and it works fine. The transmission downshifts into 3rd perfectly never having the problem on the downshift.
Some days it upshifts perfect into drive without doing this at all.
I have owned the truck since new, and it now has 150,000 miles. I changed the filter and put in Amsoil transmission fluid about 60,000 miles ago.
The fluid is very clean and looks new, with no hint of burning or looking old.
My guess is a valve inside the transmission isn't working correctly and is causing this problem. The only additional clue I have is it seems worse when its cold.